A highly bent fragment of Crithidia fasciculata kinetoplast DNA.
نویسندگان
چکیده
منابع مشابه
Replication of kinetoplast DNA in isolated kinetoplasts from Crithidia fasciculata. Identification of minicircle DNA replication intermediates.
The kinetoplast DNA (kDNA) of trypanosomes is comprised of thousands of DNA minicircles and 20-50 maxicircles catenated into a single network. We show that kinetoplasts isolated from the trypanosomatid species Crithidia fasciculata incorporate labeled nucleotides and support minicircle DNA replication in a manner which mimics two characteristics of minicircle replication in vivo: 1) the minicir...
متن کاملReplication of the kinetoplast DNA of Leishmania tarentolae and Crithidia fasciculata.
1. Replicating kinetoplast DNA networks from both Crithidia fasciculata and Leishmania tarentolae have an equilibrium density in ethidium bromide— CsQ which is less than that of covalently closed non-replicating networks. After a few hours of chase, these networks assume the covalently closed posi-. tion in the gradient. 2. Pulse-labeled minicircles isolated, from sonicated networks band in the...
متن کاملChanges in Organization of Crithidia fasciculata Kinetoplast DNA Replication Proteins during the Cell Cycle
Kinetoplast DNA (kDNA), the mitochondrial DNA in kinetoplastids, is a network containing several thousand topologically interlocked minicircles. We investigated cell cycle-dependent changes in the localization of kDNA replication enzymes by combining immunofluorescence with either hydroxyurea synchronization or incorporation of fluorescein-dUTP into the endogenous gaps of newly replicated minic...
متن کاملIsolation and characterization of kinetoplast DNA networks and minicircles from Crithidia fasciculata.
7. Cook, J. R. 1968. The cultivation and growth of Euglena, in Buetow, D. E., ed., The Biology of Euglena, Academic Press, New York 1, 243-314. 8. Cooper, T. G. & Beevers, H. 1969. P-oxidation in glyoxysomes from castor bean endosperm. J . Biol. Chem. 244, 3514-20. 9. Cramer, M. & Myers, J. 1952. Growth and photosynthetic characteristics of Euglena gracilis. Arch. Mikrobiol. 17, 384-402. 10. Da...
متن کاملStructure of discontinuities in kinetoplast DNA-associated minicircles during S phase in Crithidia fasciculata
Kinetoplast DNA (kDNA) is a novel form of mitochondrial DNA consisting of thousands of interlocked minicircles and 20-30 maxicircles. The minicircles replicate free of the kDNA network but nicks and gaps in the newly synthesized strands remain at the time of reattachment to the kDNA network. We show here that the steady-state population of replicated, network-associated minicircles only becomes...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1986
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)67383-1